Young Dental Pulp Stem Cells (DPSCs) and Tooth Regeneration
A study in dogs showed that dental pulp stem cells (DPSCs) may be useful for regenerating dying permanent teeth in younger teeth.
Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model.
J Int Med Res. 2019 Jul 31:300060519862094. doi: 10.1177/0300060519862094. [Epub ahead of print]
Huang Y1, Tang X1, Cehreli ZC2, Dai X1, Xu J1, Zhu H1.
- Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine, Department of Pediatric Dentistry, Jiangxi, PR China.
- Department of Pediatric Dentistry, Louisiana State University Health Sciences Center School of Dentistry, New Orleans, LA, USA.
To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model.
Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disinfection and pulpotomy, autologous deciduous pulp tissue was transplanted into the root canals (n = 30); as controls, contralateral teeth were treated in accordance with the recommendations of the American Association of Endodontists. Radiographic examinations were performed immediately before transplant, as well as 3 and 6 months after transplant. At the 6-month examination, root samples were collected and histological and immunohistochemical analyses were used to examine tissue regeneration.
Radiographic analysis showed no significant differences in most histopathological parameters examined; however, apical diameter reduction was greater in the experimental group. Histological and immunohistochemical analyses showed that the canal walls of the experimental group had newly formed dentin-like tissue with dentinal tubules, while the control group had cementum-like deposits along the canal wall and apical foramina.
Autologous transplantation may be useful for regeneration of dental pulp in necrotic young permanent teeth.