This paper identifies culture conditions to differentiate dental pulp stem cells into bone-like cells in anticipation of future treatment options.
Berberine Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Activating EGFR-MAPK-Runx2 Pathways.
Pathol Oncol Res. 2019 Oct 9. doi: 10.1007/s12253-019-00746-6. [Epub ahead of print]
Xin BC1, Wu QS2, Jin S2, Luo AH3, Sun DG1, Wang F4.
- Department of Cariology and Endodontology, Qingdao Stomatological Hospital, Qingdao, 266001, People’s Republic of China.
- Department of Stomatology, Tai’an City Central Hospital, Tai’an, 271000, People’s Republic of China.
- Department of Stomatology, Guizhou Provincial People’s Hospital, Guiyang, 550002, People’s Republic of China.
- Department of Pharmacy, Qingdao Stomatological Hospital, No.17, Dexian Road, Shinan District, Qingdao, 266001, People’s Republic of China. [email protected]
Similar to the mesenchymal stem cells (MSCs), dental pulp stem cells (DPSCs) also have pluripotent differentiation characteristic and may be more ideal for tissue regeneration, especially in tooth regeneration engineering. However, bacterial infection may be a powerful obstacle. Berberine (BBR), known with antibacterial effects, was recently found to play functions in bone formation through promoting osteogenic differentiation from pluripotent stem cells. However, whether BBR also function in DPSCs osteogenic differentiation has not yet been reported. Primary DPSCs were isolated from dental pulp tissues extracted from human impacted mandibular third molars, and identified by flow cytometry for cell surface antigen molecules. A dexamethasone osteogenic medium was used to induce DPSCs osteogenic differentiation. BBR (1 μM and 5 μM) was pre-added to into medium, and then cell proliferation, spheroid formation and osteogenic differentiation capacities of DPSCs were analyzed, as well as the underlying molecules modulation mechanism. Flow cytometry identified that CD44, CD90, CD81 and CD105 positively expressed in the isolated hDPSCs, with CD34 and CD45 negatively expressed. BBR enhanced the cell proliferation of hDPSCs in a dose-dependent pattern, and promoted dexamethasone-induced osteogenic differentiation via enhancing Runx2 transcription factor activity followed by upregulating osteogenesis markers expression, whereas the adipogenic differentiation of hDPSCs was suppressed dramatically by BBR. The EGFR and MAPK pathways were activated by BBR, and inhibitors for these pathways significantly suppressed the osteogenic differentiation promotion of BBR. These results have revealed a novel mechanism that berberine might promote hDPSCs osteogenic differentiation through activating EGFR-MAPK-Runx2 signaling pathways.