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Wound Healing Using Human Stem Cells

This paper indicates that substances secreted from human stem cells can help to improve wound healing.

ABSTRACT

Human Adipose-Derived Stem Cell Secreted Extracellular Matrix Incorporated into Electrospun Poly(Lactic-co-Glycolic Acid) Nanofibrous Dressing for Enhancing Wound Healing.

Polymers (Basel). 2019 Oct 3;11(10). pii: E1609. doi: 10.3390/polym11101609.

Tang KC1, Yang KC2, Lin CW3, Chen YK4, Lu TY5, Chen HY6, Cheng NC7, Yu J8.

Author information

  1. Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan. [email protected].
  2. School of Dental Technology, Taipei Medical University, Taipei 106, Taiwan. [email protected].
  3. Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan. [email protected].
  4. Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan. [email protected].
  5. Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan. [email protected].
  6. Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan. [email protected].
  7. Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan. [email protected].
  8. Department of Chemical Engineering, College of Engineering, National Taiwan University, Taipei 106, Taiwan. [email protected].

Abstract

Wound dressing, which prevents dehydration and provides a physical barrier against infection to wound beds, can improve wound healing. The interactions between extracellular matrix (ECM) and growth factors is critical to the healing process. Electrospun nanofibers are promising templates for wound dressings due to the structure similarity to ECM of skin. Otherwise, the ECM secreted by human adipose-derived stem cells (hASCs) is rich in growth factors known to enhance wound healing. Accordingly, we propose that the PLGA nanofibrous template incorporated with hASCs-secreted ECM may enhance wound healing. In this study, PLGA nanofibrous matrixes with an aligned or a random structure were prepared by electrospinning. Human ASCs cultured on the aligned matrix had a better viability and produced a larger amount of ECM relative to that of random one. After 7 days’ cultivation, the hASCs on aligned PLGA substrates underwent decellularization to fabricate cECM/PLGA dressings. By using immunohistochemical staining against F-actin and cell nucleus, the removal of cellular components was verified. However, the type I collagen and laminin were well preserved on the cECM/PLGA nanofibrous matrixes. In addition, this substrate was hydrophilic, with appropriate mechanical strength to act as a wound dressing. The L929 fibroblasts had good activity, survival and proliferation on the cECM/PLGA meshes. In addition, the cECM/PLGA nanofibrous dressings improved the wound healing of surgically created full-thickness skin excision in a mouse model. This hASCs-secreted ECM incorporated into electrospun PLGA nanofibrous could be a promising dressing for enhancing wound healing.

 

PMID:31623334

DOI:10.3390/polym11101609